High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection.
نویسندگان
چکیده
A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.
منابع مشابه
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ورودعنوان ژورنال:
- The Analyst
دوره 136 22 شماره
صفحات -
تاریخ انتشار 2011